Amino Beads

Amino Beads

MACS (ImpetiCbead, IMB referred beads), in recent years developed a new immunological techniques, the unique height advantage and immunological reaction curing agents will specifically bind to one to immunological basis, penetrated into all areas of pathology, physiology, pharmacology, microbiology, biochemistry and molecular genetics, which has been more widely used in immunoassays, cell separation, purification of biological macromolecules and molecular biology.

Principle:

Using core - shell synthetic polymer synthesis surface four superparamagnetic iron oxide-containing polymer cover, good stability, can be post-labeled substance, the use of these substances surface features such as an amino group, a carboxyl group, a thiol group, etc. antibody covalently or non-covalent coupling can be used to bind the corresponding antigen, so that under the applied magnetic field to attract do directional movement, so as to achieve the separation, detection, purification of target genes, proteins, cells, microorganisms and the like.

Main applications:

1, Gene purification: use of the silicon bead surface may be reversible under certain conditions of the adsorption and dissociation characteristics of DNA / RNA can be used to purify the gene, genes isolated object, such as genomic DNA, material DNA, viral DNA, whole blood genomic DNA, RNA and the like.

2, protein purification: the use of a carboxyl group, an amino group, streptomycin, avidin, a protein sulfhydryl cross-linking corresponding antibodies can specifically and effectively isolated to the corresponding protein.

3, cell purification: this area has become a major application of MACS.

4, microbial enrichment: the use of microbial corresponding antibody beads can efficiently be reversible enrichment corresponding microorganisms.

Features:

YR-NH2-01

1. The amino abundant:> 150μmol / g;

2. Operating performance: beads uniformly dispersed superparamagnetic, magnetic response time <30s;

3. Inter-batch stability and good repeatability: uniform particle size, polydispersity index <0.2, was monodisperse;

YR-NH2-02

1. Amino-rich content: ~ 1000μmol / g;

2. Operating performance: beads uniformly dispersed superparamagnetic, magnetic response time <10s;

3. Inter-batch stability and good reproducibility, carboxyl content between batch CV <5%;

The goal of high volume substances binding, nonspecific adsorption is low: dedicated to separation and purification field.